Background: Airborne disease is any disease caused by a microorganism and transmitted through the air. Aim of the study: Was to assess awareness of patients attending to Chest Clinic regarding airborne diseases in Benha City. Research Design: Descriptive research design was utilized to conduct this study. Setting: This study was conducted at Chest Outpatient Clinic affiliated to Benha University Hospital. Sample: Simple random sample was used this study and they were be chosen according to inclusion criteria; patients which their age is above 21 years. The total sample size was 303 patients. Tools: Two tools were used. Tool I: A structured interviewing questionnaire which consisted of three parts to assess demographic characteristics and medical history, knowledge of patients regarding airborne diseases and reported practices regarding prevention of airborne diseases. Tool II: Scale to measure the attitude of patients about airborne diseases. Results: 43.9% of the studied patients were age ranged from 40 to less than 50 years old, 44.2% of them had primary education, 51, 5 % of them had average knowledge level about airborne diseases, 70,6% of them had unsatisfactory practices level and 50, 8 % of them had positive total attitude level regarding airborne diseases. Conclusion: There were highly statistically significant relations between the studied patients' total knowledge scores, total practices, and their total attitude scores. Recommendations: Educational program should be developed and implemented for patients to improve their knowledge, practices and attitude toward prevention of airborne diseases.