Background: Antibiotic stewardship is the effort to measure and improve antibiotics prescribing by specialists and antibiotic usage by children. Aim of the study: This study aimed to assess effect of educational program on pediatric nurse's performance regarding to antibiotic stewardship. Research design: A quasi-experimental design was utilized in the study. Setting: The study was conducted in Pediatric Intensive Care Units (PICUs) at Benha University Hospital. Sample: A convenient sample included all available nurses (50) who working in the previously mentioned setting. Tools of data collection: Tool (I) A Structure Interviewing Questionnaire sheet to assess nurse's characteristic and their knowledge regarding antibiotic stewardship programs. Tool (II) An observational questionnaire Format to assess nurse's practice regarding to antibiotic stewardship programs. Tool (III) An observational questionnaire Format to assess nurse's attitude regarding antibiotic stewardship programs. Results: There was a positive correlation between pediatric nurse's knowledge, practice and attitude scores pre and post implementation of the educational program Conclusion: The educational program was effective in improving the level of pediatric Nurse's performance regarding to antibiotic stewardship. Recommendations: Enhancing nurse's performance regarding antibiotic stewardship programs through in service training program in PICUs. Further studies; repeating the study on large sample to generalize the results