Background: Professional ethics inform nurses to provide care to their patients based on ethical regulations and rules and achieved best patients' outcome. Aim: This study aimed to enhancing nurses' knowledge and practice toward professional ethics and its effect on patient outcome (patients' satisfaction). Design: A quasi-experimental design was utilized. Setting: Conducted in all medical departments /units at Benha University Hospital. Subjects: Two subjects: I- nurses group: a proportion a location sample was taken from the above-mentioned setting and consisted of 30% from total number of nurses, the final number was (60) and II- patients group: convenience sample of patient according to formula was (74). Tools: Three tools were used to collect data: I. Professional ethics knowledge questionnaire, II. Professional ethics observational checklist, III. Patient outcome assessment (patient satisfaction). Results: Most of nurses (78.3%&73.3%) had satisfactory knowledge level regarding professional ethics at immediate post and follow up program phases (after 3 months) respectively than preprogram phase was (40%). Most of nurses (75%& 73.3%) had a good practice level regarding professional ethics at immediate post program and follow up phase (after 3 months) respectively than preprogram phase was (43.3%). The majority of studied patient (90% & 83.8%) had satisfied level regarding professional ethics at immediate post program and follow up phase (after 3 months) respectively than preprogram phase (63.5%). Conclusion: Implementation of educational program on professional ethics for nurses was effective There was a highly statistical significance positive correlation between nurses' total knowledge, total practice regarding professional ethics and their total patient outcome (patient satisfaction). Recommendations: In-service education and training programs must be continuous process for refreshing nurses' knowledge and practice regarding professional ethics and conduct study about professional ethical application strategies focusing on nurses that enhancing patient's outcome.