Background: Self-Learning Package is one of the most suitable teaching methods for learners. Oxygen therapy is a lifesaving, medical intervention in the management of hospitalized children. The nurses have a very important role in this regard as they should monitor carefully and regularly children who relate to oxygen therapy. Aim of this study was to evaluate the effect of implementing self-learning package about oxygen provided to children on nurses' performance at Pediatric Intensive Care Unit. Research design: A quasi-experimental research design was utilized to conduct the study. Settings: The current study was conducted at the Pediatric Intensive Care Units at University Hospital & Specialized Pediatric Hospital at Benha city. Subjects: A convenient sample of all nurses (70) and a purposive sample of children (70) on oxygen. Tools of data collection: Two tools: tool (1) A structured interviewing questionnaire and tool (2) observational checklists. Results: More than half of nurses had low total score of knowledge regarding oxygen provided to children pre-implementation of the self-learning package, while more than two thirds of them had high total score of knowledge post-implementation of self-learning package. Also, about three quarters of nurses had unsatisfactory total score of practice regarding oxygen provided to children pre-implementation of the self-learning package, while more than three quarters of them had satisfactory total score of practice post-implementation of self-learning package. Conclusion: Self-Learning Package was effective in improving nurses' performance regarding care of children undergoing oxygen therapy. Recommendations: Provision of continuing educational program in order to update nurses' knowledge and enhance their practice regarding care of children on oxygen at pediatric intensive care unit.