Background: Use of emergency contraceptive methods reduce risk of unintended pregnancy that contributes in reducing maternal morbidity and mortality. Aim: The aim of the study was to evaluate effectiveness of educational intervention on knowledge and attitude of maternity nurses regarding emergency contraception. Setting: The study was conducted in Obstetrics and Gynecological (Department, Outpatient Clinic and Emergency) at Benha University Hospital. Design: A quasi experimental design was utilized. Sampling: A convenient sample included 80 Maternity nurses. Tools of data collection: Two tools were used, Tool I: A structured self-administered questionnaire which consisted of 2 parts, Part (1):  General characteristics of the studied nurses, Part (2): Maternity nurses' knowledge assessment sheet and Tool II: Maternity nurses' Attitude assessment sheet. Result:  There was a highly statistically significant improvement in relation to maternity nurses' knowledge and attitude regarding emergency contraception at post intervention phase compared to pre intervention phase. As well as, There was a highly statistical significant positive correlation between total knowledge and total attitude scores regarding emergency contraception at pre- and post- intervention phase. Conclusion: The educational intervention had a positive effect on the improvement of maternity nurses' knowledge and attitude regarding emergency contraception. Recommendations: Applying continuous in-service training program for maternity nurses to improve their knowledge and attitude regarding emergency contraception.