Background: Basic life support entails the recognition of sudden cardiac arrest and the immediate administration of cardiopulmonary-resuscitation and defibrillation with automated external defibrillator. These interventions can double or triple survival rates of victims with sudden cardiac arrests. The aim of this study: Was to evaluate the effect of a basic life support training program on university nursing students. Research design: A quasi-experimental design was used. Setting: This study was conducted at faculty of Nursing in Benha University. Sample: Simple random sample of 110 forth year students. Tools: Two tools were used; Tool I: Structured interviewing questionnaire included two parts A): Demographic characteristics of the studied students. B): Knowledge assessment of the studied students. Tool II: Observational checklist to measure the studied students' level of basic life support practice. Results: 77.3% of the studied students had good total knowledge post implementation compared by 15.5% pre implementation of the program and 80.0% of the studied students had a satisfactory level of total practices post implementation compared by 30.9% pre implementation of the program. Conclusion: The implemented training program succeeded to improve the studied students' levels of knowledge and practice about basic life support. Recommendations: Continuous training of basic life support throughout nursing education curriculum during the academic years and after graduation on regular basis and evaluation of application of automated external defibrillator devices in order for nursing students to be familiar with it.