Background: Uterine fibroid affect woman in reproductive age, increased hazard of premature delivery and blood loss at delivery. Aim of the study: To assess maternity nurses' knowledge, instructions and attitudes regarding uterine fibroid Study design:  A descriptive design was utilized. Setting: The study was conducted at Obstetrics and Gynecological departments. Operating room and Obstetric outpatient in Benha University hospital. Sample: A convenient sample included 50 maternity nurses. Tools of data collection: Three tools were used for data collection, tool(I): Self-administered questionnaire to assess knowledge regarding uterine fibroid. Tool (II):  Assessment sheet to assess nurses' instructions regarding uterine fibroid and tool (III): Modified likert scale to assess attitude regarding uterine fibroid. Results: More than half of the studied nurses had poor knowledge and negative attitude and also about three fifth of the studied nurses had unsatisfactory instructions   regarding uterine fibroid. Conclusion: Less than half of studied nurses had good, average knowledge and positive attitude and about also two fifth of the studied nurses had satisfactory instructions regarding uterine fibroid. Recommendation: Developing a periodical training program for nurses working at Obstetrics and Gynecological departments to enhance and updated with the necessary knowledge, practices about the recent trends of uterine fibroid.