Background: Breastfeeding is the cornerstone of infant and young child survival, nutrition, development and  also, for maternal health. Aim: The research aimed to assess knowledge, practices and attitude of post-partum mothers regarding breast feeding during COVID-19. Research design: A descriptive design was utilized. Setting: This study was conducted at postnatal unit of  Obstetrics & Gynecological Department in Benha University hospital. Sampling: A purposive sample included 240 breast feeding mother. Tools of data collection: Four tools: were used First Tool: A structured interviewing questionnaire which consisted of three parts: Socio-demographic characteristics of mothers, obstetric history and history related to breastfeeding, Second tool: Mother's knowledge regarding breast feeding during COVID-19. Third tool: Observational checklist to assess the practice of mothers regarding breast feeding during COVID-19. Fourth tool: Modified Likert Scale to assess the attitude of mothers regarding breast feeding during COVID-19. Results: More than one half of the studied mothers had poor knowledge regarding breast feeding during COVID-19, more than one half of the studied mothers had satisfactory practices regarding breast feeding during COVID-19, while about two thirds of the studied mothers had positive attitude regarding breast feeding during COVID-19. Conclusion: More than  one half  of the studied mothers had poor knowledge regarding breast feeding during COVID-19  and more than one half of the studied mothers had satisfactory practices regarding breast feeding during COVID-19.  Also, about two thirds of the studied mothers had positive attitude regarding breast feeding during COVID-19. Also, there was a highly positive statically significant correlation between total knowledge, total practices and total attitude regarding breast feeding during COVID-19, so the study results answered the study questions.  Recommendation: Developing training programs for mothers to improve their knowledge and maintain optimal breastfeeding practice.