Background: Polycystic ovary syndrome is associated with hormonal disturbance and adverse cosmetic, reproductive, metabolic and psychological consequences, resulting in reduced quality of life. Research aim: Was to assess knowledge and quality of life of women with polycystic ovary syndrome Research design: A descriptive research design was utilized. Research setting: The current research was conducted at Obstetric and Gynecological outpatient clinic in Benha University Hospital. Research sampling: A purposive sample included 231 women. Tools of data collection: Two tools were used. Tool (I): A structured interviewing questionnaire to assess women's general data, menstrual, obstetrics and family history, present history of studied women about polycystic ovary syndrome and women's knowledge regarding polycystic ovary syndrome. Tool (II): Polycystic ovary syndrome quality of life questionnaire. Results: More than three quarters of studied women had poor level of knowledge and more than two thirds of studied women had low quality of life. There was a highly significant positive correlation between women's total knowledge and quality of life scores (p-value 0.001). Conclusion: Nearly most of the studied women had low level of knowledge regarding polycystic ovary syndrome. More than two thirds had low quality of life. Recommendations: Implementing integrated health education to support the women with polycystic ovary syndrome to improve the quality of life.