Background: Infertility is a global public health issue with a high impact on individuals of both sexes and society. Infertility is ranked as the 5 the highest serious global disability. Aim of study: Was to assess the quality of life among infertile women in Benha City. Research design: Descriptive research design was used to conduct this study. Setting: The study was conducted at Obstetrics and Gynecology Outpatient Clinic in Benha University Hospital. Sample: A convenience sample was used, it included 120 infertile women. Tools: Two tools were used; I: A structured interviewing questionnaire to assess; A) Socio-demographic characteristics of the studied women. B) Past history of the studied women. C) Knowledge of the studied women about infertility. D) Reported practices to improve fertility. II: Scale to assess quality of life among infertile women. Results: 59.2% of the studied women were aged ranged from 30 to less than 40 years old, 43.3% of the studied women had university education, 10.0% of the studied women had good knowledge level about infertility, 51.7% of the studied women had satisfactory reported practices regarding infertility, and 53.3% of the studied women had poor quality of life. Conclusion: Only tenth of the studied women had good knowledge level about infertility, more than half of the studied women had satisfactory reported practices regarding infertility, more than half of the studied women had poor quality of life. There were highly statistically significant relation between the studied women total quality of life score, total knowledge score and total reported practices score. Recommendations: Develop health educational program for the infertile women to improve and update the most current knowledge, practices and quality of life about infertility.