Background: Immune thrombocytopenia is an autoimmune disease characterized by a low platelet count (less than 100 u 109 /L) and pediatric patients may develop bruising or mucosal bleeding. The concept of health-related quality of life was first proposed decades ago and has been successfully applied in a variety of contexts. Aim: This study aimed to improve the quality of life of mothers and their children with immune thrombocytopenia. Design: A quasi-experimental design was utilized. Setting: The study was conducted at Benha Specialized Pediatric Hospital which affiliated to secretariat of specialized medical centers (in outpatient clinics and hematology ward) at Benha city. Sample: A purposive sample of children with Immune thrombocytopenia aged from 2 to less than 18 years and their mothers were recruited. Tools of data collection: Four tools were used to conduct the study. Tool (I): Structured Interviewing Questionnaire which included 2 parts; Part (1) Personal characteristic of studied mothers.  Part (2): Mother's knowledge regarding ITP. Tool (II): Medical Data Sheet which included 2 parts; Part (1): Personal data of the studied children. Part (2): Previous and current medical history. Tool (III): Mothers' reported practice to assess mothers' care given to their children with immune thrombocytopenia which include nose bleeding, wound care, bathing, teeth brushing, mouth care, skin care and hair care and administration of oral medications.  Tool (IV): Scale of pediatric quality of life (Kids ITP tools) to assess the quality of life of children with Immune thrombocytopenia. Results: Less than quarter (22.0%) of the studied mothers had satisfactory level of reported practice in pre-guidelines phase, while (86.0% & 80.0%)   of them had satisfactory level of reported practice toward improving the quality of life of their children with thrombocytopenia in post- guidelines and at follow-up phase. Mean child scores of PedsQL was 48.18±14.93 pre guidelines and 60.32±11.22 post guidelines phase. Less than half (40.0%) of the studied children had positive quality of life in pre-guidelines phase, while (68.0% & 64%) of them had positive quality of life in post-guidelines phase and follow-up phase. Conclusion: The designed guidelines succeed to improved mother's knowledge and reported practice regarding ITP and children's quality of life. Recommendations: More researches are required for training of mothers, about the proper care of children with immune thrombocytopenia and raising their awareness about the nature of disease and right practices should be done in daily different situations.