Two potting experiments were conducted in the Faculty of Agriculture and Natural Resources, Aswan University, Aswan, Egypt during the two seasons of 2022/2023 and 2023/2024 to investigate the influence of vermicompost on tuberose Polianthes tuberosa L plant. A completely randomized design were used in four replicates, each contain three pots. Four vermicompost treatments (control, 10, 20 and 30% of used media) were applied. Morphophysiological characteristics i.e. leaves number, leaves length, fresh and dry weight of leaves, leaf area, days to flowering time, spike length, spike fresh and dry weight, rachis length, number of florets/plant, flower diameter, number of new bulbs/plant, fresh weight of hill, diameter of new bulbs and fresh weight of new bulb, as well as chemical composition as N,P, K, carbohydrate contents and polyphenols were recorded for tuberose from bulb sprouting to harvest. The obtained results indicated that the plants received vermicompost 30% produced the highest values of all vegetative growth parameters. Applying 30% vermicompost registered the highest values of all flowering traits. The best results for bulb production and the higher percentages of N,P, K, and carbohydrate as well as chlorophyll a&b, carotenoids and polyphenols were also associated with the 30% vermicompost treatment as comparing with the other levels. Generally, the obtained results showed that applying vermicompost improved the growth characteristics, flowering parameters, bulb production and chemical constitutes of tuberose under the ecological conditions of Aswan region.