The current investigation was conducted at the ornamental plant nursery and laboratory of the Faculty of Agriculture and Natural Resources, Aswan University, Aswan, Egypt during the two seasons of 2022/2022 and 2023/2024, with the aim of studying the impact of treating with different concentrations of marine algae extract as soil drenching on the growth, flowering, and bulb production aspects as well as chemical components of tuberose (Polianthes tuberosa L.) plants. This experiment was arranged as a randomized completely design with four replicates. Five algae extract treatments namely; control or untreated plants, 0.5, 1.0, 1.5 and 2.0 g/l were applied. It was found that treating tuberose plants at a concentration of 1.5 g/l coincide with obtaining maximum values for the vegetative growth measurements i.e. leaves number/ plant, leaves length, fresh and dry weight of leaves and leaves area. Treating plants with 1.5 g/l produced the maximum values of the flowering attributes like spike length, spike fresh and dry weight, rachis length, number of florets/plant, flower diameter, and the least days to flowering time. Also, the best results regarding the bulb production and the content of N,P, K, pigments, total carbohydrates as well as polyphenols were recorded with 1.5 g/l marine algae extract as comparing with the other treatments. In general, the obtained results showed that applying seaweed extract improved the growth, flowering, and bulb production as well as chemical characteristics of tuberose plants under the environmental conditions of Aswan region.