Lake Qarun (Fayoum Province, Egypt) is characterized as a highly eutrophic lake. Management of zooplankton resources in the lake received slight attention. A total of 22 and 23 zooplankton taxa were recorded at some lake stations (from Q1 to Q10) during the autumn and winter seasons respectively. During the autumn, the recorded zooplankton taxa were divided into four groups namely: Rotifera (47.63%), Copepoda (29.23%), Protozoa (18.4%) and Meroplankton (4.73%). Concerning the winter, the recorded zooplankton taxa were divided into five groups namely: Copepoda (36.02%), Rotifera (30.72%), Protozoa (23.48%), Meroplankton (9.69%) and Cladocera (0.038%). The Multiple regression analysis indicated that Shannon diversity index values were significantly influenced by the temperature, transparency, EC, pH and dissolved oxygen. This investigation provides informative information about the dynamics and biodiversity of zooplankton communities in the lake and their interactions with certain environmental factors. The results explored the variations in species diversity, richness, and evenness among the evaluated lake stations. Stations with higher diversity indices tend to have higher species richness and a more even distribution of individuals among species. Understanding these variations can provide valuable insights into the ecological health and dynamics of another similar aquatic ecosystem.