Pulicaria crispa is a medicinal and aromatic plant that is distributed around the world, particularly in arid and sub-tropical regions. It has been widely utilized in the treatment of a variety of diseases since ancient times. In this study, a remarkable variety of endophytic fungi and bacteria were isolated from different parts of P. crispa. Fourteen endophytic fungal species belonging to seven genera (Cladosporium, Chaetomium, Volutella, Emericella, Aspergillus, Penicillium, and Ulocladium) were recorded. The relative abundance of genus Aspergillus was 40.5% (recorded the highest count of genera). Volutella ciliata registered the highest fungal species count with a relative abundance of 16.2%. Five bacterial species belonging to the genus Bacillus including Bacillus sianensis, B. safensis, B. subtilis, B. altitudinis, and B. vallismortis were isolated. B. sianensis which colonized roots represented the highest relative abundance percent (32 %). This is the first study of the microbes' diversity inside the tissue of the medicinal plant P. crispa.