Phragmites australis may be considered one of the plants used a lot in treating areas contaminated with phosphate, it contains a strong microbial community that can be used in treating areas contaminated with phosphates including Enterobacter cloacae, as used in this study. This study aims to use Enterobacter cloacae isolated during the summer of 2019 from Phragmites australis from the Kima drain located in Ezzbet El- Nahda in Kima, Aswan Governorate, for removing phosphate in synthetic wastewater. These bacteria were tested for their phosphate removal efficiency using the MIC assay. The highest growth of Enterobacter cloacae in the synthetic wastewater supplied with a concentration of (900) mg/l of phosphate after 72 hours of treatment was (0.350), and the change in pH after 72 hours of treatment was (7.1), and the remaining phosphate in Synthetic wastewater after 72 hours of biological treatment with Enterobacter cloacae and using a carbon source such as glucose and pH (7.9) was (530.33) mg/l with a removal rate of (41.074%). Thus, we found that Enterobacter cloacae achieved high efficiency in removing phosphates from the synthetic wastewater, despite the presence of phosphates in high concentrations close to the MIC (Minimum Inhibitory concentration). Thus, Enterobacter cloacae use pollutants as nutrients and can be used to treat environments contaminated with phosphates.