Extensive use of antibiotics and their mismanagement significantly contribute to the emergence of soil microorganisms that are resistant to antibiotics, especially in the root zone. In this study, twenty-one isolates of Rhizobium leguminosarum biovar vicia were isolated from three cultivars of faba- bean that is cultivated in the El Shenab area of Aswan Governorate. For these isolates, morphological, cultural, and biochemical characteristics were examined. 21 isolates of the soil bacterium Rhizobium leguminosarum were screened for their response to three widely used antibiotics by using the minimum inhibitory concentration (MIC) test. The isolates were grown in a yeast mannitol ager (YMA) medium, which contains different concentrations (100 – 1000 PPM) of three antibiotics commonly used in Egypt (amoxicillin, cefotaxime, and tetracycline) individually. Six resistant isolates were obtained. The isolates (40A, 40B, and 843B) were the most resistant of the six isolates to antibiotics with high efficiency under the concentration of 1000 PPM for the three antibiotics. The percentage of tolerance and inhibition of the antibiotic for these three isolates was Amoxicillin (72%, 65%, and 45%), Cefotaxime (60%, 55%, and 47%), and Tetracycline (65%, 55%, and 51%) respectively. The three isolates showed high efficiency in breaking down antibiotics. These isolates can be used in agricultural farms that contain antibiotics.