Introduction: As a cost-efficient and powerful pesticide, rodenticide, and fumigant, aluminium phosphide (ALP) has been widely utilized. It can result in widespread suicide poisoning and high mortality due to its ease of availability. ALP's active ingredient is phosphine (PH3). The human body will suffer total damage as a result of exposure to PH3. Aim of work: The goal of the current study was to examine the autopsy results from various human organs that had been poisoned with ALP in the Medico-legal Department, Benha, Qalyubiyah, Egypt. Methodology: In this work, 10 control cases and 10 chemically confirmed autopsy cases were included and examined in gross detail. Samples of tissue from various organs were stained with hematoxylin-eosin and examined microscopically. Results: This study includes a number of gross autopsy observations that have been made. Upon concentrating on the pathological changes in the major organs, we discovered substantial PH3-induced damage in many systems, particularly the stomach, duodenum, lung, and kidney. We also noticed extensive myocardial, splenic, and hepatocellular deterioration in the heart, spleen, and liver tissues. Conclusion: This study considered that these characteristic abnormalities were a possible indicator of PH3 poisoning and partially explained the substance's deadly nature (inhibition of mitochondrial oxidative phosphorylation) and that may contribute to a better understanding of PH3 toxicity in both forensic and clinical conditions.