Using LC-HRESIMS to perform metabolomic profiling on the secondary metabolites of the Dalbergia sisso (Roxb.) ex DC leaves ethanol extract, a number of physiologically active components belonging to several chemical groups were identified. In addition to previously described neoflavonoid and isoflvonoids, which were most prevalent and showed both qualitative and quantitative variation, four other types of flavonoid were found in the plant for the first time. They comprised twenty-one amino acids, other nitrogenous chemicals, five coumarins, eight phenolic acids, five anthocyanins, two alkaloids, and 46 flavonoids. Additionally, the stilbene glycoside E-3,4,5'-Trihydroxy-3'-glucopyranosylstilbene was found in the D. sisso extract. Along with primary metabolites (Cytidine-3', 5'-cyclicmonophosphate), there are three growth regulators ((+/-)-cis, trans-abscisic acid, beta-indoleacetic acid, and Gibberelin A3). Screening study led to the identification or preliminary characterization of 111 compounds. D. sissoo ethanolic leaf extracts successfully protected rabbits from infectious diarrhoea when given orally. This is demonstrated by blood measures (Hb, Lymphocytes, Monocytes, and Esinophil) and weight parameters.The ethanolic leaves extract of D. sissoo significantly prolonged the onset of diarrhea and reduced the number of wet and total stools at doses of 300 mg/kg and 500 mg/kg as compared to the negative control so, It is highly recommended to use D. sisso as a natural antidiarrheal drug.