Rheumatoid arthritis (RA) is a chronic inflammation of the immune system that similarly affects the synovial joints. Atopic dermatitis (AD) is a chronic skin condition that is characterized by a complicated interaction of repeated exposures, host factors, and hereditary factors. Interleukin 10 (IL-10) is an immune-regulatory cytokine that is commonly utilized in RA to reduce the inflammatory response. An allelic variation in the IL-10 gene's promoter region may play a role in autoantibody production control. This study aims to identify Polymorphisms in the IL-10 gene in RA and AD patients and sequence the IL-10 gene fragment to elucidate the polymorphism (IL10-1082A/G) and determine the possible relationship between IL10 gene polymorphism and pathogenesis of RA and AD. Twenty-five blood samples were collected from RA Egyptian patients / 17 healthy control samples and 19 blood samples were collected from AD Egyptian patients / 12 healthy control. DNA has been extracted from blood samples and subjected to Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) test for the determination of IL-10 genotype. PCR products were sent for sequencing. Genotyping revealed that both the patient and control groups had the same IL-10 allele; sequencing results showed silent mutation with no effect in the amino acid sequence. Our results showed that there is no association between IL10-1082 A/G single nucleotide polymorphism and RA or AD pathogenesis. Environmental factors may have more effect on the pathogenesis of RA and AD.