Background: Acetamiprid (ACE) is a neonicotinoid insecticide, known in the trade market as: Mospilan, Assilan and Chipco. It is used to get rid of sucking insects. Its intake might cause toxic effects on the cerebellum. Objectives: The study aimed to determine the pathological effects of acetamiprid on the cerebellar cortex of male adult albino rats and the role of both Curcumin and Vitamin C in altering those pathological effects. Materials and methods: Forty male adult albino rats were used in this study. The rats were divided into four groups (n=10 each): group I (control) and received corn oil, group II (treated with acetamiprid orally), group III (treated with acetamiprid + Curcumin orally), group IV (treated with acetamiprid + Vitamin C orally). Cerebellar tissue samples were used for histopathological examination using light and transmission electron microscopy. Morphometric and statistical studies were done to measure Purkinje cell (PC) number and the thickness of the granule layer. Results: There was a significant structural damage of cerebellar cortical tissue in group II. Group III showed restoring of the pathological structure of cerebellar cortex compared to group II. Group IV showed less improvement compared to group III. Conclusion: Curcumin and vitamin C could have a protective effect against neurotoxicity induced by acetamiprid via their antioxidant property.