Background: Acrylamide is a widespread chemical that is used in industries and its exposure is wide from certain cooked foods like potato chips. Nevertheless, this chemical is approved to be dangerous to many organs. 
Objectives: revealing the effect of acrylamide on the histological structure of the urinary bladder. 
Materials and methods: this research was done on a number of 30 adult male rats, which were divided equally into 3groups: control group I: Without treatment, group II: treated with acrylamide 5mg orally for 6 days, group III: treated with acrylamide 5mg for 28 days. The rats were then sacrificed and the bladder was taken and prepared for histological and Immuno-histochemical study.  
Results: After 6 days, the rats showed destruction of epithelium, congestion, and muscle thinning, then after 28 days, the epithelium showed edema, more congestion, and more thinning. With Caspase-3, the rats showed more reaction after 6 days than after 28 days, with tumor necrosis factor (TNF); the expression of the gene increased after 28 days.  
Conclusion: Acrylamide is toxic to the bladder even for a short duration and should be avoided as possible.