Abstract: Background: The implementation of Team Strategies and Tools to Enhance Performance and Patient Safety (Team STEPPS), reduce nursing errors, increase team awareness, clarify team roles, resolve conflicts, improve sharing information, enhance quality of care, and patients' safety. Purpose: To evaluate the effect of Team STEPPS intervention on nurses' knowledge and their performance related to teamwork. Methods: A quasi-experimental two groups' study research design was utilized. A convenient sampling technique was used to recruit 80 nurses from the study setting. Instruments: Two instruments were used: Team STEEPS Knowledge questionnaire, and Team performance observation instrument. Results: Post- intervention, study group showed remarkable improvement of STEPPS approach knowledge (95% Vs 0.0%) and performance (85% Vs 0%) pre-intervention. Regarding dimensions of STEPPS approach, the results showed no statistically significant difference between study and control group in all dimensions before intervention. However, post intervention, the results show high statistically significant difference in all STEPPS dimensions. Conclusion: Nurses who received STEPPS approach intervention had higher level of knowledge and performance related to teamwork. Recommendations: The study recommends reinforcement of Team STEPPS to sustain quality of care and patients' safety.