Background: perceived stress in the workplace is a growing concern globally, particularly in high-stress professions such as nursing. The effects of workplace stress on nurses and healthcare organizations can indeed be significant, including illness, absenteeism, and reduced work performance. Purpose:  was to examine the effect of resilience training program on perceived stress among acute care nurses. Design: Quasi-experimental two groups' research design was used. Setting: it was conducted in the acute care units at Beni-Suef University Hospital. Sample: Simple random sampling technique was used to recruit 160 nurses from acute care units. Instruments: The Modified Connor-Davidson Resilience Scale and perceived stress scale were used. Results: Study group had very high resilience level after implementation of the program (60%). Nearly half of the study group had a very low perceived stress level after implementation of the program (56.3%). Conclusion: Resilience training is an effective method that helps nurses to better handle stressful situations in their workplace. Recommendations: Nurse Leaders should continue to promote resiliency to nursing staff by reinforcing protective factors such as good time management, problem solving and decision making, effective communication and supportive professional relationships. Build positive and supportive work culture through monthly meetings, conferences to voice their problems, opinions, and needs.