Abstract: Background: Pediatric endoscopy is a widespread diagnostic and therapeutic procedure in children. However, it causes significant anxiety to children and their families. Purpose: To evaluate the effect of designed video based learning strategy on endoscopy- related anxiety among children and their parents. Design: A quasi-experimental research on two groups using pre and posttest design has been implemented. Setting: Endoscopy Unit of Menoufia University Hospital at Shebin El-Koom City, Menoufia Governorate, Egypt. Sampling: A purposive sample of 80 children undergoing endoscopy procedure and their parents. Instruments: Five instruments were utilized to collect data (A structured interview questionnaire, State-Trait Anxiety Inventory (STAI) Likert scale, Modified Yale Preoperative Anxiety Scale (m-YPAS), Measurement of saliva- cortisol levels and Procedural properties observational sheet). Results: There was a significant decrease in the total mean score of parental state and trait anxiety in the study group on post intervention (88.90 ± 14.52) than on pre intervention (118.35 ± 16.97). There was a significant reduction in total mean score of children's anxiety in the study group on post intervention (40.20 ± 8.85) than on pre intervention (75.66 ± 11.11). Children in the study group had significantly lower saliva-cortisol levels, shorter duration of sedation, endoscopy and recovery than children in the control group. Conclusion: The video-based learning strategy was successful in reducing endoscopy- related anxiety among children and their parents. Furthermore, the length of all stages of the procedure was dramatically lowered when the stress associated with endoscopy in children was reduced. Recommendation: Children should be provided with strategies to help them feel less anxious before to their endoscopy.