The adoption and acceptance of artificial intelligence (AI) by nurses, doctors, patients and decision makers is essential for its successful application. Healthcare professionals still have varied attitudes and perception concerning the use of AI technology, despite the fact that there is growing interest in this area. Aim: To assess nurses' perspective and attitude toward utilization of artificial intelligence in health care. Methods: A descriptive research design with consecutive sample of 200 nurses from Qena University Hospitals (QUHs) was utilized. Instruments: Two instruments were used; nursesꞌ perception of AI scale and nursesꞌ attitudes towards AI scale. Results: The results showed that 92.0% of participants had moderate perception toward using AI in health care and 64% of them had positive attitude toward usage of AI in health care. There were highly statistically significance difference between nurses' perception toward AI, gender and social status. Likewise there were statistically significance difference between nurses' attitude toward AI, and age, qualifications, social status and years of experience. Conclusion: The majority of nurses had moderate perception and positive attitude toward usage of AI in health care. Recommendations: Health policy makers must develop strategies to increase readiness of the institutions to apply AI and increase the capabilities of nurses for appropriate use of AI through well trained. Physicians, nurses, and patients should adopt the utilization of AI in the work field.