Germs are everywhere and can attach to hands and other items through daily contact. Hand hygiene is one of the most crucial measures to get rid of germs and prevent their spreading. Students with blindness (SWB) are at a higher risk of infection due to using their hands to discover the world around them. The community health nurses are required to estimate students with blindness' knowledge about hand hygiene to care for these students; and support them to live and work in the community healthfully. For that, this study aimed to assess the knowledge of students with blindness regarding hand hygiene. This was conducted through a descriptive cross-sectional study at Alnour School in Mansoura City on 39 students with blindness recruited conveniently; by a structured interview questionnaire to identify demographic characteristics, and assess knowledge of the students with blindness regarding hand hygiene. All students with blindness had a poor level of knowledge regarding hand hygiene. It is recommended to design health educational programs to raise students with blindness' awareness regarding hand hygiene.