Background: The administration of chemotherapy is a high-risk practice which entails numerous risk factors with a
high error index. The safe and standard handling of antineoplastic drugs can reduce the effects of occupational exposure
and promote safe behaviors in nurses. Thus, the present study aimed to evaluate the effect of chemotherapy safety
standard guidelines on oncology nurses' knowledge, and practice. Methods: A quasi-experimental design one-group
pretest posttest was utilized. Sample/Setting: A convenient sample of all available nurses working in Sabha oncology
centers Libya who met inclusion criteria was selected. Study tools: The data were collected by using a demographic data
form and nurses 'knowledge assessment questionnaire regarding the standard guidelines for working with antineoplastic
drugs, and a standard checklist for examining their skills in this regard. were analyzed by descriptive (mean and standard
deviation) and inferential statistics (t-test) and Pearson's correlation coefficient) in SPSS 26. Results: the data revealed
that, there were High statistically significant differences regarding nurses' knowledge and practice mean scores were
found between the pre-test, and post-test (34.61±7.42,47.83±2.22 for knowledge, & 23.00±17.5029.00±26.22for
practice) respectively, where P values of Wilcoxon test P = 0.000. Conclusions: A significant improvement in nurses'
knowledge and practice was found after implementation of the teaching program which supported the study hypotheses.