Background: To keep the airway open and avoid difficulties from secretion retention, one of the most frequent
operations in newborn intensive care units is endotracheal tube aspiration. Suction should not be routine and should be
done only when a knowledgeable, experienced, and competent nurse is needed to avoid complications. Aims: Evaluate
the effectiveness of educational program on nurses' knowledge & practices regarding neonatal endotracheal tube
suctioning. Design: A quasi-experimental one-group pretest/posttest research design was utilized in this study. Setting:
the study was carried out in neonatal intensive care unit affiliated to Mansoura university children hospital. Sample: A
convenience sample of 60 nurse over a period of six months were included. Tools for data collection: A structured
questionnaire sheet, and observational checklist were used for data collection Results: The results showed that total
knowledge and performance scores were higher in the posttest and follow-up than in the pretest, with statistically
significant differences. In addition, there was a positive linear correlation between nurses' overall knowledge of neonatal
endotracheal tube aspiration and practice scores. Conclusion: The study concluded that implementation of a training
program improved nursing staff's knowledge and practice of neonatal endotracheal tube suctioning. Recommendations:
Researcher recommended that. Nurses should engage in in-service training to advance their knowledge and improve
their practices related to neonatal endotracheal suctioning.