Background: Environmental cleaning of the operating rooms through housekeeping services such as maintenance of safe,
clean and well-groomed environment keeps the environment in a good sanitation and free from potential infection. Aim:
Assess the knowledge and performance of nurses and cleaners regarding environmental cleaning in operating rooms.
Sample: A ample of 154 nurses and 48 cleaners participated in the study. Design: A mix between quantitative (crosssectional
design) and qualitative design. Setting: This study carried out at operating rooms departments in which major
surgical procedures are being implemented at Mansoura University Hospital Tool: Three tools were used in this study,
self-administrated demographic and occupational, characteristics questionnaire, knowledge assessment questionnaire,
observational checklist. Results: regarding operating room cleaning process, 53.2 % of the studied nurses showed fair
level of knowledge, whereas 85.4% of the studied cleaners showed good level of knowledge. Vast majority of both
nurses and cleaners showed improper performance regarding operating room cleaning process. There was a significant
association between studied nurses' total knowledge with age, education, and years of experience. There was not any
association between studied nurses' total performance with their demographic characteristics. Conclusion: Majority of the
studied nurses showed poor knowledge and improper practice regarding operating room cleaning process whereas
cleaners showed good knowledge but have improper practice. Significant association documented between studied
nurses' total knowledge with age, education, and years of experience. Recommendation: Provide a protocol for
healthcare workers about operating rooms environmental cleaning, continuous training and periodic assessment of
healthcare workers' performance related to environmental cleaning.