The present study aims to delve into the comprehensive chemical composition of chia seeds, with a specific emphasis on elucidating the detailed profile of amino acids. Furthermore, the study sought to evaluate the potential therapeutic efficacy of chia seed protein in modulating blood glucose levels in male diabetic rats. The chemical composition of seeds was as follows: moisture (5.55 %), crude fiber (18.99 %), ash (1.92%), crude protein (18.16 %), total lipids (33.59 %), and total carbohydrate (21.79%). The amino acids composition was as mentioned next: glutamic acid 20.6% followed by arginine 12.47%, meanwhile the aspartic and leucine recorded 9.57 and 7.76% respectively. After 30 days of treatment with chia seeds protein, hyperglycemic rats experienced a significant decrease in glucose levels from 244.17 mg/dl to 119.24 mg/dl. Additionally, triglycerides decreased from 119.74 mg/dl to 81.34 mg/dl, and the total cholesterol level decreased from 126.41 mg/dl to 98.13 mg/dl. Furthermore, the hyperglycemic group's LDL-C and HDL-C were compared. Consequently, the protein found in chia seeds may be advantageous for individuals with diabetes.