Beta
ManageSearchChatDiscover
344787

CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.

Article

Last updated: 26 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Agricultural Biotechnology and related articles researches

Abstract

: Twenty-six isolates used in qualitative screening of Cellulase done in two media CMC-Congo red dye plate and 1% PASC (phosphoric acid swollen cellulose) plate. Quantitative Enzymes activities were tested in two fermentation methods SSF (solid state fermentation) and SmF (Submerged fermentation). SmF was detected at 2,3,4,6 and 7 days in Mandel's Media with CMC 0.5%. Moreover, SSF was carried out by using straws (Rice and wheat) for all isolates. The cellulase activities measured as (FPase, CMCase and β-glucosidase). In this study positive correlation coefficient in enzymes activity between SSF and qualitative screening in (CMC & PASC) plate. Higher correlation have been detected between PACS and SSF rice straw was about (0.595**) for β-Glucosidase. After all these tests we can compare enzyme production by SmF and SSF. Data showed SmF production for high yield and high quality. The cellulase genes, Chb gene encoding cellobiohydrolase 2 (CBH2) and Bgl gene that encoding endo-β-gluconase (BGL1) were present in all Trichoderma isolates. 

DOI

10.21608/mjab.2024.271332.1016

Keywords

Trichoderma spp, Cellulase genes, SSF, SmF, CMCase, FPase, β, glucosidase, CBH2, BGL1

Authors

First Name

Muhammad

Last Name

ELsobky

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt

Email

mohamed.elsobky@agr.menofia.edu.eg

City

-

Orcid

0000-0002-2818-6842

First Name

Khalid

Last Name

Abdel-lateif

MiddleName

Salah

Affiliation

Department of Genetics, Faculty of Agriculture, Menoufia University

Email

k_dein2001@yahoo.com

City

-

Orcid

-

First Name

Abdelmegid

Last Name

Fahmi

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt

Email

abdelmegidfahmi@yahoo.com

City

-

Orcid

-

First Name

Abdelfatah

Last Name

Elzanaty

MiddleName

-

Affiliation

Genetics Department Faculty of agriculture Menoufia Univesity Shibin El kom

Email

zanaty1966@gmail.com

City

Menoufia

Orcid

-

First Name

Ragaa

Last Name

Eissa

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt

Email

profeissa@yahoo.com

City

-

Orcid

-

Volume

9

Article Issue

2

Related Issue

46947

Issue Date

2024-02-01

Receive Date

2024-02-15

Publish Date

2024-02-29

Page Start

23

Page End

38

Print ISSN

2357-0768

Online ISSN

2735-3532

Article File

MJAB_Volume 9_Issue 2_Pages 23-38.pdf

PDF . 1.4MB

Link

https://mjab.journals.ekb.eg/article_344787.html

Detail API

https://mjab.journals.ekb.eg/service?article_code=344787

Order

3

Type

original papers

Type Code

1,460

Publication Type

Journal

Publication Title

Menoufia Journal of Agricultural Biotechnology

Publication Link

https://mjab.journals.ekb.eg/

MainTitle

CORRELATION BETWEEN QUALITATIVE AND QUANTITATIVE CELLULASE ENZYMES ACTIVITIES IN SOME TRICHODERMA SPP.

Details

Type

Article

Created At

26 Dec 2024