:The present study sheds light on the mportance of zinc oxide nanoparticles in various fields: Such as biomedical effects, electrochemistry, catalysis, sensors, biomedicine, pharmaceutics, healthcare, cosmetics, food technology, textile industry, mechanics, optics, electronics, space industry, energy science and optical devices, metal oxide nanoparticles display a very strong and widely applicable impact. In this respect we Preparation of Zinc oxide nanoparticles from zinc acetate by two sol-gel methods.and determined Characterization of zinc nanoparticles via: X-ray Diffraction method , FTIR analysis . TEM analysis of the ZnO-NPs. The XRD pattern of the as-prepared sample shows that the crystalline phase of ZnO had not been induced. On the other hand, the annealed powdered samples exhibit a long range order of sharp Bragg peaks. The miller indices for each peak have been indexed to the standard hexagonal wurtzite structure of ZnO. The ZnO nanoparticles annealed from 300 to 600°C displayed similar Bragg peak patterns. The obtained FTIR spectrum of all the ZnO samples produced through various methods proved that the final product formed is ZnO in all the two cases. Through TEM analysis It was observed that the morphology of the ZnO-NPs have grown in a near-hexagonal shape, the image shows that the powder consists of quasi-spherical nanoparticles. The majority of ZnO-NPs measured fell in the range of 30 to 50 nm in diameter with an average size of 40 nm in diameter. This is in close agreement with the results obtained from the powder XRD measurements. TEM measurements are based on the visible grain boundaries, while the XRD calculation measures the extended crystalline region that diffracts X-ray coherently. The result optaind can be summarized in the following points: 1-    Antioxidant activity of zinc oxide nanoparticles, there was a concentration dependent increase in antioxidant activity was observed. The data showed that ZnO NPs were further highlighted by the quenching of DPPH free radicals. The absorbance values ​​for ZnO NPs (A) ranged from 49.25 to 53.79% and 58.24 to 59.54% for ZnO NPs (B) compared to the absorbance values ​​for ascorbic acid from 95.2 to 95.9%, and the order of effectiveness is followed as follows: Ascorbic acid > ZnO NPs (B) > ZnO NPs > ZnOs (NPs) > Zn O NPs (NPs) > ZnO NPs (NPs) > 2-    Antimicrobial activity of  zinc oxide nanoparticles, Data showed the inhibition zones of ZnO NPs  (A) and ZnO NPs  (B) against that the ZnO NPs  (A) and ZnO NPs  (B)   have sufficient antimicrobial  activity. For all nine gm (-) , three gm (+) organisms , three fungi and yeast,  it inhibited the growth and the growth inhibition is dose dependent. As the dose is increased the zone of diameter of inhibition is also increased.