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322270

PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM

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Last updated: 26 Dec 2024

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Abstract

ABSTRACT: Siderophores are ferric ion specific chelating agents produced by bacteria and fungi growing under low iron stress. The role of these compounds is to scavenge iron from the environment and to make the mineral, which is an essential metal, available to the microbial cells. Azotobacter sp.  isolate Azo-4 isolated from Sadat City ,Egypt showed positive result for siderophores production by Chrome Azurol Sulfonate (CAS) assay. The organism was subjected to various biochemical tests and 16S rRNA analysis; results indicated that the isolated fragment sequence (~1.3) Kbp is homologous to Azotobacter chroococcum strain KCA1 16S ribosomal RNA gene. Deferrated medium concentration induced the siderophores production (45 µM). Hydroxymate siderphore was extracted and the antagonistic activity of partially purified siderphore was tested against bacterial pathogens Bacillus stbtlius and Salmonella sp.,in vitro. Result showed that the Azotobacter sp. under study is a good producer of siderophores, which can be beneficial for its antagonistic activity towards different pathogens as biocontrol agent.

DOI

10.21608/mjab.2015.322270

Keywords

Azotobacter Chroococcum, Hydroxamate siderophores, 16srRNA, SEM, Biocontrol

Authors

First Name

Shimaa

Last Name

Dwedar

MiddleName

-

Affiliation

(1) Industerial Biotechnology Dept., Genetic Engineering and Biotechnology Institute, Sadat city

Email

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City

-

Orcid

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First Name

Marwa S. Abdel-

Last Name

Hamid

MiddleName

-

Affiliation

) Microbial Biotechnology Dept.2, Genetic Engineering and Biotechnology Institute, Sadat city university, Egypt

Email

-

City

-

Orcid

-

First Name

Doaa

Last Name

Keshk

MiddleName

-

Affiliation

(3) Genetic Engineering and Biotechnology Institute , Agriculture Research Center, Giza- Egypt

Email

-

City

-

Orcid

-

First Name

A.A.

Last Name

Haroun

MiddleName

-

Affiliation

(4) Division of Chemical Industries Research, National Research Center, Dokki, Egypt.

Email

-

City

-

Orcid

-

First Name

A.F.

Last Name

El Baz

MiddleName

-

Affiliation

(1) Industerial Biotechnology Dept., Genetic Engineering and Biotechnology Institute, Sadat city

Email

-

City

-

Orcid

-

Volume

40

Article Issue

2

Related Issue

44009

Issue Date

2015-04-01

Receive Date

2015-01-06

Publish Date

2015-04-01

Page Start

409

Page End

417

Print ISSN

2357-0768

Online ISSN

2735-3532

Article File

MJAB_Volume 40_Issue 2_Pages 409-417.pdf

PDF . 868.1kB

Link

https://mjab.journals.ekb.eg/article_322270.html

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https://mjab.journals.ekb.eg/service?article_code=322270

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original papers

Type Code

1,460

Publication Type

Journal

Publication Title

Menoufia Journal of Agricultural Biotechnology

Publication Link

https://mjab.journals.ekb.eg/

MainTitle

PRODUCTION OF HYDROXAMATE SIDEROPHORES BY AZOTOBACTER CHROOCOCCUM BACTERIUM

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Article

Created At

26 Dec 2024