ABSTRACT:Many Trichoderma aggregate species are of economic interest because of their ability to produce hydrolytic enzymes. In this study,twenty isolates of Trichoderma were obtained from lignocellulosic agriculture wastes-rich soilcollected from different Egyptian locations (Sadat, Tala, Abo Hamad, Belbeis, Zagazig, Mansoura, Belqas,Kafer-Elshikh, Bella, Tanta, Borg El Arab, Banha, Kafr Shoker, Qalyoub, Shebien Elqanater, Damanhur,Abu al-Matamir, Damietta, Kafr al-Battikh, and Kafr Saad). These isolates were firstidentified morphologically based on conidiophore branching type and conidium morphology. Seven isolates were found to belong toTrichoderma Viride: TM4, TM5, TM9, TM13, TM16, TM29, and TM42; four isolates classified as Trichoderma Koningii: TM6, TM8, TM18 and, TM19 and nine isolates were identified as Trichoderma Harizianum: TM23, TM30, TM31, TM33, TM35, TM36, TM41, TM44, and TM45.