This study investigated the air quality in poultry house for ammonia concentration along with N%, pH, and moisture in litter material and the related poultry health. The poultry litter was used for biochar production for mitigating ammonia emissions. Accordingly, 20 chicks of one day age were used without any treatment as a control group for 35 days. After this period the poultry litter was used in biochar production at 450 ° C. Two broiler groups (each of 20 chicks) were constructed as 10% biochar (10%BC group) and 20%biochar added to the litter (20%BC group). The concentration of ammonia in air, as well as litter moisture in control group were significantly high comparing with biochar groups. Although feed consumption by the control group was more than biochar groups, results showed significant decrease in the body weight gain in control group compared to the biochar groups, especially 10%BC. Feed conversion ratio was highly recorded in control group than biochar groups. Moreover, serum total protein and albumin contents were significantly decreased in control group compared to biochar groups, especially 10%BC. However, levels of liver nitric oxide and interlukin-6 (IL-6) was significantly increased in control group compared to the two biochar groups. On contrast, interlukin-10 (IL-10) recorded significant decrease in control group compared to both biochar groups. Consequently, addition of biochar 10% and 20% to the bedding material resulted in decreasing ammonia emission in the air environment of poultry house which in turn lead to improvement in the chicken health, especially with 10%BC group.