Considering the difficult challenges facing the hotel sector in terms of hotel employees' eco-friendly behavior and environmental organizational culture, green training becomes a crucial issue for the hotel industry to address in order to meet employee needs. However, Green training for employees is essential to hotels' success. Research on the relationship between eco-friendly conduct and green training through the moderating influence of the hotel industry's environmental organizational culture is lacking. The current study aimed to reveal the influence of green training GT on hotel employees' eco-friendly behavior EEB, taking into account the role of environmental organizational culture EOC. To examine the proposed model of the study with a random sample of 450 valid responses collected from employees in five-star hotels in Cairo and using structural equation model analyses, The PLS-SEM approach was used to analyze data by WarpPLS software version 7.0. Through the moderating effect of environmental organizational culture, the results demonstrated the beneficial effects of employee green training on eco-friendly behavior. The findings showed that there is a positive correlation between GT and EEB, and there is a positive correlation on the connection between GT and EOC. Furthermore, EOC positively correlates with both GT and EEB, additionally; EOC has a positive moderating impact on the connection between GT and EEB. So, the study enhances green organizational behaviors by demonstrating that organizations promoting green work engagement encourage employees to reciprocate by adopting green citizenship behaviors. This study also offers practical guidance for the hotel sector and managers to promote green training and hotel employees' eco-friendly behavior for environmental organizational culture. Findings revealed that an employee's perception of training, rewards, and recognition significantly contributes to increasing employees' levels of EOC. The findings indicated that GHRM practices are not implemented in Egyptian hotels in Cairo because of management and staff obstacles. These findings have ramifications for both academics and hoteliers. In light of these findings, theoretical and managerial implications as well as future research directions are discussed. This study may be among the few that examines the influence of green training on hotel employees' eco-friendly behavior taking, into account the role of environmental organizational culture.