Amniotic membrane (AM) is a biologically viable structure with many properties that made it a popular choice in regenerative medicine. It has low immunogenicity, reepithelialization effects, anti-inflammatory, nontumorigenic and antimicrobial properties. These pleiotropic capabilities are due, in part, to its ability to synthesize and release biologically active chemicals such as growth factors. Amniotic membrane extract (AME) is one of the many modifications that are done on the amniotic membrane offering a much simpler and convenient approach. Despite being perceived as a single entity; amniotic membrane has many subregional differences proved histologically and so it stands to reason that the content of the biological factors differs as well according to the region. Knowledge on specific factors could facilitate the decision on which region to select for a specific application. Transforming growth factor-beta and Hepatocyte growth factor are of particular interest given their intimate involvement in all phases of wound healing.
AIM:
The aim of this study was to assess the total protein content and concentration of Hepatocyte Growth Factor (HGF) and Transforming Growth Factor-Beta1 (TGF-β1) in amniotic membrane extract for their therapeutic uses and compare their concentrations between placental and reflected regions of AM.