Acute liver failure (ALF) is a life-threatening clinical syndrome manifested by jaundice, ascites, hepatic encephalopathy, and a bleeding tendency in a patient without previously known liver disease. To date, the Management of severe ALF is through liver transplantation which carries several disadvantages. So, there is an urgent need for novel therapeutic options. Stem cells are unspecialized immature progenitor cells that can self-renewal and differentiate into multiple cell lines. Platelet-rich plasma (PRP) is an autologous plasma solution that expresses various growth factors.
AIM OF THE WORK:
The aim of the present work was to evaluate the biochemical, histological, and immunohistochemical changes induced by stem cells alone and in combination with PRP on rat liver treated with Acetaminophen.
PATIENTS AND METHODS:
Five groups of male rats were treated as follows: Group 1 was the control group, group2 was given CCl4 in olive oil (2.5 ml/kg, IP), Group 3 was administer edacetaminophen (APAP) in sterile saline (1gm / kg, IP),group 4 was given APAP then were injected with stem cells (3 x106 cells / ml: 1ml / kg) 24 h after APAP injection, group 5 was injected by APAP then were injected with stem cells (3 x suspended in PRP 24 h after APAP injection. Blood samples were taken for determination of serum alanine aminotrans ferase (ALT), aspartate trans aminase (AST), and albumin, liver specimens were collected for histopathological examination.