The study objectives were to examine the effects of difenoconazole (Score 25%) being stored at 54°C for 21 days under accelerated conditions and estimation the impact the effects of subchronic exposure to fungicide on male albino rats. The findings showed that difenoconazole was reasonably stable while stored under accelerated conditions at 54±2°C for 21 days. Regarding the subchronic effects of difenoconazole, it was obvious that both doses 1/20 and 1/40 of LD50 increased the concentration of ALT, AST, cholesterol, and triglycerides, and decreased the total protein concentration. With respect to histopathological effect on liver, difenoconazole at dosage 1/20 induced severe dilatation and congestion in the portal vein with few inflammatory cells infiltration in the surrounding tissue of the portal area. Also, diffuse inflammatory cells, infiltration and few fibroblastic cells proliferation , degenerative changes in the hepatocytes with inflammatory cells infiltration surrounding the dilated central vein.