This work was conducted to study This study was conducted at the farm of the Faculty of Agriculture, Menoufia University, to find out the most important nematodes that infect the most important commercial cultivars of tomatoes in Egypt, as well as assessing the sensitivity of these cultivars to root-knot nematodes and conducting control experiments using safe methods. Meloidogyne is one of the most numerous and dangerous parasitic nematodes that attack tomato plants under open field conditions, followed by Pratylenchus. Super estrain B cultivar considered one of the most sensitive tomato cultivars to Meloidogyne javanica compared with rest of the studied cultivars. While the most resistant variety is Vacolta38. Chemical nematicide Vydate 24% L is more effective on root-knot nematodes that infect tomatoes, followed by the bio-zeid, then Nemafree, and finally Nemastop. The treatment with Vesicular-arbuscular mycorrhizal (VAM) fungi (Glomus mosseae) gave the highest reduction percentage in root-knot nematode Meloidogyne javanica J₂, followed by Humic acid, then Nofatrien, and finally Salicylic acid. The study concluded that there are safe and effective alternatives against root-knot nematodes that give better results than the effect of the chemical pesticide, as the triple treatment {Humic acid + G. mosseae +Vydate (half dose)} gave the highest reduction percentage in root-knot M. javanica J₂ infected tomato plants with an overall mean by (90.0%), followed by triple treatment triple treatment (Humic acid + G. mosseae + Bio-zeid (half dose)) by (88.0%), as well as improving the vegetative characteristics of tomato plants compared to the chemical pesticide Vydate, as it is safe for humans, animals and plants and less expensive than chemical nematicides., As for the two treatments that had the highest effect on root-knot nematodes J2, double treatment (G. mosseae + Vydate (half dose)), which gave a death rate of root-knot nematodes J₂ reaching (81.6%),. Triple treatment (Humic acid + G. mosseae +Vydate (half dose)) improved the vegetative and root characteristics of tomato plants, as it led to an increase in plant height, shoot weight and root weight in percentages (+50.6% & +28.1% & 143.2%) respectively compared to the control . It also led to a decrease in root gall index by (-80.0%) compared to the control. As for the triple treatment (Humic acid + G. mosseae + Bio-zeid (half dose)), it improved the vegetative and root characteristics of tomato plants, as it led to an increase in plant height, shoot weight and root weight in percentages (+53.0% & +29.0% & 148.1%). respectively compared to the control. It also led to a decrease root gall index by (-80.0%) compared to the control. Therefore, this study recommends the use of environmentally safe methods for humans, animals and plants, as well as effective on root-knot nematodes, as an alternative to the excessive use of chemical nematicide, which cause significant damage to the public health of humans and animals.