Eggplant plants are a vital cheap summer vegetable. Eggplant plants are known to be extremely susceptible to root-knot nematodes infection which causes severe damage that leads to yield losses. six eggplant cultivars i.e. SNO-8-1, 108-3-1, BPCL-1, SCR-2, CLA-11-2 and SWD-13-1 were Evaluated for susceptibility to Meloidogyne spp. the cultivar (108-3-1) is the most susceptible cultivar and the lowest one is the BPCL-1 cultivar. The chemical analysis using HPLC to study the differentiation between tolerant and susceptible eggplant cultivars to Meloidogyne spp. recorded that the highest accumulation of total phenol compounds is detected (BPCL-1) cultivar, whereas the lowest concentrationse record in (108-3-1) cultivar The genetic variation among the six eggplant cultivars was studied by using 10 primers for the SSR region, and the results showed the presence of genetic diversity among the tested cultivars, especially the EEMS15 and EEMS16 primers. And adding different bioagents (Bacillus megaterium, Pseudomonas fluorescens, Azospirillum brasilense, Paenibacillus polymexa and Trichoderma harzianum), as well as bio product (nemaless) compared with chemical control (fenamiphos) against root-knot nematodes, Meloidogyne spp., results showed that Fenamiphos achieved the highest reduction in nematode parameters, and among all treatment within biocontrol agent B. megaterium show the highest reduction of nematodes parameters. using different colors of polyethylene sheets i.e. (transparent, Black, Blue and Red) results showed that the transparent color sheet was the best one in reducing the nematode parameters, while the lowest value was recorded with the black color sheet. The best program of integrated biological and physical and chemical methods was used to control Meloidogyne spp. by using with transparent sheets + Bacillus megaterium applied before nematode inoculum + fenamiphos. It reduced nematode parameters.