The aim of this studying to evaluate yield performance for 38 promising long staple genotypes with two check varieties, Giza 95 and Giza 98 in Trial A at Sids agriculture research in 2022 season, and select the best 22 promising genotypes from Trail A to evaluate these selected promising genotypes in Trial B in 2023 season, with the two check Variety at five locations (Beni-sueif, El-Fayoum, Assiut, Sohag and Luxor Governorates). Results of trail A indicated that, 30 promising genotypes belonging to 17 crosses were superior the two check varieties in seed and lint cotton yields. Two promising genotypes which No. 32 and No. 33 belonging a promising cross (G.91 x G.90) x S109 were surpassed the best check variety Giza95 in boll weight (BW). Results of trail B showed that, locations significantly differed for all the studied traits, The genotypes mean squares were significant for seed cotton yield (SCY) and boll weight (BW), the genotypes environment interactions mean squares were significant for (LCY) and highly significant for seed cotton yield (SCY) and boll weight (BW). Mean performance across all locations showed that, seven promising genotypes which No. 1 (G.95 x [(G.91 x G.90) x G.80]), 5 (G.95 x G102), 8 (G.90 x A108), 12 (G.72 x [(G.83 xG.80) x G.89]), 19 and 20 ((G.91 x G.90) x S108 (24202)) and No. 21 ((G.91 x G.90) x S109) were significantly more yielded on the best check variety Giza-95 in seed cotton yield across all locations, and all the same crosses were equal to the check varieties in boll weight trait. Broad sense heritability was obtained for seed cotton yield (LY), lint yield (SCY), and boll weight (BW), which was 14.33, 7.34, and 9.59%, respectively, indicating the presence of low amount of genetic variance because of environmental factor. From results of Trial A and Trial B we can concluded that, five promising crosses which were G.95 x [(G.91 x G.90) x G.80], G.90 x A108, G.72 x [(G.83 xG.80) x G.89], (G.91 x G.90) x S108 (24202) and (G.91 x G.90) x S109 may be considered as a promising materials for future breeding programs to develop and isolate high yielding varieties of Egyptian cotton for upper Egypt conditions