The present investigation was carried out in the Experimental Farm at El-Gemmeiza Agriculture Research Station, (ARC), El-Gharbia Governorate, Egypt, during 2018 and 2019 summer seasons with aims to produce F1 crosses (by hybridization between maize and teosinte) superior in productivity and drought tolerance. Also, to estimate heterosis, combining ability and genetic variance of the crosses and their parents. In 2018 summer season, seven different genotypes (four females and three males) belong to different species of Zea were used in the present study. Four maize genotypes (S.C. 130, S.C. 168, T.W.C. 321 and T.W.C. 352) belong to Zea mays were used as female parents (Lines), while three teosinte genotypes (Gemmeiza 3, Gemmeiza 4 and Sakha 1) were belonged to Zea mexicana (Euchlaena mexicana) were used as male parents (testers). The four females and three males were crossed according to (line × tester) mating design to produce 12 F₁ crosses outlined by Kempthorne (1957). In 2019 summer season, all tested genotypes, i.e. seven parents (four maize and three teosinte) as well as their 12 F₁ crosses were grown under three irrigation systems i.e., irrigation every 12 (normal), 18 (moderate drought) and 24 days (stress drought) to determine the mean performance, heterosis and general and specific combining ability of all tested genotypes.  At each irrigation system, the seven parents and 12 crosses were arranged in a randomized complete block design with three replicates.