ABSTRACT: Leaf rust caused by Puccinia triticina Eriks is considered one of the most three important worldwide disease of wheat causing regular and significant losses over large geographical areas. Four bread wheat cultivars i.e. Gemmiza-7, Misr-1, Sids-1 and Sids-12 were assessed for their disease parameters, yield measurements, physiological and histological features. Assessments were carried out during 2012/13 and 2013/14 growing seasons, under realistic field conditions of Kafr El-Hamam Agricultural Research Station in El-Sharkia governorate, ARC, Egypt. According to their infection responses, Misr-1 and Sids-12 were considered as resistant cultivars. Meanwhile, Gemmiza-7 and Sids-1 were susceptible. Different correlations between disease parameters and some physio-histological features were performed. Resistant wheat cultivars recorded the lowest values of disease percentages, average coefficient of infection (ACI) and area under disease progress curve (AUDPC) values. These results were related to the free phenol contents and average number of stomatae/mm2 by a direct correlation. These relations were reversed in total and conjugated phenols, peroxidase activity, chlorophyll contents, distance between stomatal rows, schlerenchyma thickness, and laminar bundles parameters. All the aforementioned correlations were completely reversed in the susceptible wheat cultivars. Also, a correlation between chlorophyll values and yield/plot was observed. The resistant Sids-12 wheat cultivar recorded the highest values of chlorophyll content with the subsequent highest values of yield/plot which compatible for the lowest percentage of yield loss (0.32 %), during 2012/13 growing season. While, the highest percentage of yield loss (23.73%) was recorded by the susceptible Sids-1 wheat cultivar, during 2012/13 growing season. Selected parameters used in the present work can be considered as a base for future investigations of leaf rust status on some commercial Egyptian bread wheat cultivars.