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321145

IN VITRO CULTURE OF SOME GUAVA CULTIVARS

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Last updated: 04 Jan 2025

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Abstract

ABSTRACT: The experiment was carried out in the Plant Tissue Culture Lab.of the Tissue Culture Lab.of Hort.Dept., Faculty of Agric., Minoufiya Univ., Shebin El-Kom in cooperation with Tissue Culture Lab., of Plant Biotechnology Dept., Genetic Engineering and Biotechnology Research Institute, Sadat City University, during the years 2010 to 2013 to investigate the procedures of tissue culture technique for clonal propagation of Banaty, Sabahy and Mobaker (guava cultivars). After surface-sterilization, the plant materials (shoot tips) were cultured in Murashige and Skoog (MS-1962)mediumsupplemented with various plant growth substances that favour shooting.The best disinfestation procedure obtained when the plant materials were immersed in 0.5% sodium hypochlorite for 6-8 minutes, followed by dipping in 70% ethanol for 1- 4 minutes, then in 0.3% mercuric chloride for 1 minute. Varietal differences appeared by applying different treatments of the three guava cultivars. BA at 1.0 mg/l gave the best effect on shoot production per explant of the three guava cultivars. The better effects on shoot production were obtained when IBA at 0.02 to 2.0 mg/l was combined with BA at 1-2 mg/l. Varietal differences appeared by changing the concentrations of the growth substances used.

DOI

10.21608/mjppf.2015.321145

Keywords

Surface-sterilization, proliferation, guava, tissue culture

Authors

First Name

M.R.M.

Last Name

Rabeh

MiddleName

-

Affiliation

Hort.Dept., Fac. Agric., Minuofiya Univ., Shebin El-Kom.

Email

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City

-

Orcid

-

First Name

S.A.

Last Name

Ahmed

MiddleName

-

Affiliation

Hort.Dept., Fac. Agric., Minuofiya Univ., Shebin El-Kom.

Email

-

City

-

Orcid

-

First Name

A.A

Last Name

kasem

MiddleName

-

Affiliation

Hort.Dept., Fac. Agric., Minuofiya Univ., Shebin El-Kom.

Email

-

City

-

Orcid

-

First Name

Basma S

Last Name

.Salama

MiddleName

-

Affiliation

Hort.Dept., Fac. Agric., Minuofiya Univ., Shebin El-Kom.

Email

-

City

-

Orcid

-

Volume

40

Article Issue

1

Related Issue

43911

Issue Date

2015-02-01

Receive Date

2014-11-18

Publish Date

2015-02-01

Page Start

131

Page End

138

Print ISSN

2357-0830

Online ISSN

2735-346X

Article File

MJPPF_Volume 40_Issue 1_Pages 131-138.pdf

PDF . 1.0MB

Link

https://mjppf.journals.ekb.eg/article_321145.html

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https://mjppf.journals.ekb.eg/service?article_code=321145

Order

5

Type

original papers

Type Code

1,393

Publication Type

Journal

Publication Title

Menoufia Journal of Plant Production

Publication Link

https://mjppf.journals.ekb.eg/

MainTitle

IN VITRO CULTURE OF SOME GUAVA CULTIVARS

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Article

Created At

26 Dec 2024