The present investigation was carried out at Sakha Agricultural Research Station, Kafr El-Sheikh and clayey saline soil at the experimental farm of El-Sirw Agricultural Research Station, Agriculture Research Center, Damietta governorate (31°14′N and 31°48′E) at the northern east of the Delta, Egypt during 2021 and 2022 seasons. Four Egyptian rice genotypes i.e. Giza178, Giza179, GZ9399-4-1-1-3-2-2 as a salt tolerant varieties and Sakha 106 as salt sensitive one were used as line and three rice genotypes; IRRI147 and IR65483 (salt tolerant varieties), Egyptian Jasmine ( salt moderately salinity tolerant variety)  as tester were utilized. The parental varieties were crossed by using line & tester's analysis and twelve crosses were obtained. The F₁ and their parents were grown and evaluated under saline soil. The studied characters were yield and yield attributes. The obtained results indicated that the parents, Giza178, Giza179 and IRRI147 were the best parents for general combining ability for studied traits under saline soil conditions. Meanwhile, the crosses, Giza179×IRRI147, Giza178×IRRI147 and Giza179×E.Jasmine were the best performing crosses and could be used under salinity condition as well as are considered as the most effective for getting promising lines. Assessing the genetic distance and linkages between seven different rice types were used to perform ISSR markers to identify genetic differences that could aid in the creation hybridization and selection of breeding program.