This study was designed to evaluate the effect of certain strains of fungi (Trichoderma harzianum) and yeast (Saccharomyces cerevisiae D-47) inoculation on silage chemical composition, fermentation characteristics, and in-vitro digestibility.  Four treatments were tested i.e., control (C): corn silage without inoculants, Y: corn silage involved saccharomyces cerevisiae, T: corn silage involved Trichoderma harzianum and Y+T: corn silage involved both inoculants. Chopped whole corn was pressed into polyethylene bags (1.5 to 2 kg) using a vacuum sealer, then stored at room temperature for different ensiling times (zero time, 5 h, 10 h, 20 h, and 2, 4, 8, 14, 25, and 35 days). Inoculants had no significant effect on DM and OM, while decreased (P<0.04) significantly with ensiling time. In spite corn silage CP and NFE increased (P< 0.5) significantly with inoculants and ensiling time than the control (c), the content of CF, NDF, and ADF significantly decreased with the time of ensiling. The values of pH and NH3-N gradually decreased in corn silage with the time of ensiling.  The lactic acid concentration increased (P < 0.001) with inoculation of Yeast (Y), Trichoderma (T), or both (Y + T) and reached 39.50, 38.99, and 40.77 g/kg DM, respectively. While the acetic and butyric acid followed the opposite trend. Time of ensiling negatively correlated with the concentration of both formic and citric acids while it was positively correlated with the concentration of succinic acids.  Silages inoculation increased total bacteria (5.51, 7.69, 7.69, and 7.81 log₁₀ cfu/g DM) for the control, Y, T, and Y+T, respectively. Similarly, lactic acid bacteria significantly increased with inoculation (6.46, 6.89, 6.97 and 7.03 log₁₀ cfu/g DM) for the control, Y, T, and Y+T, respectively. Moreover, yeast count (log₁₀ cfu / g DM) increased (P<0.05) significantly with silage inoculation compared to the control silage, and the significantly highest was obtained by Y + T. The inoculation had significantly (P < 0.05) increased values of both IVDMD and IVOMD, where the best values appeared with corn silage inoculated with Y+T.
Conclusion, the inoculation of Saccharomyces cerevisiae D-47 and/or Trichoderma harzianum leading to an increase in silage quality compared with the un-inoculated silage.