In recent decades, much attention has been directed into algae-based biotechnology, encompassing wastewater treatment, biofuel generation, medicines, nutraceuticals, and environmental remediation. Optimizing algal growth conditions is crucial to maximize their potential and improve their efficacy in many biotechnological applications. This study examined the growth performance of Tetradesmus obliquus (Chlorophyta) and Synechocystis pevalekii (cyanobacteria) under various culture media (Bold's Basal medium, BG-11 medium, Khul's medium, and Zarrouk's medium), nitrogen sources (NaNO3, KNO3, NH4Cl, and peptone), and pH levels (7, 9, 11, and 13). Our research indicated that both species achieved optimal growth performance utilizing Bold's Basal medium. of these conditions, total chlorophyll concentrations of S. pevalekii and T. obliquus were significantly elevated by 27.98 and 22.28 times, respectively. The findings indicated that sodium nitrate was the most efficacious nitrogen source, markedly augmenting the optical density and chlorophyll content of both species, with OD increasing by 15.74 and 8.27 times for S. pevelekii and T. obliquus, respectively, in relation to the beginning value. Sodium nitrate was identified as the optimal nitrogen source, significantly increasing the optical density by 15.74 and 8.27 times for S. pevalekii and T. obliquus, respectively, as well as enhancing total chlorophyll content. Concerning pH niches, pH 7 was ideal for S. pevalekii, but pH 13 was most conducive for T. obliquus.