The metabolic effects of silver nanoparticles AgNPs prepared by phycocyanin extracted from Spirulina platensis on metabolic activities of economic plant Phaseolus vulgaris including  seed germination, pigments(chl. a, chl. b and carotenoids) , carbohydrates, total lipids, amino acid contents, proline, protein banding, malondialdehyde  as well as the  antioxidant enzymes activities,  superoxide dismutase [SOD], peroxidase [APX], and non-enzymatic antioxidants such as glutathione reductase (GR), catalase [CAT] have been investigated. The results demonstrated that all studied parameters seed germination, pigments, carbohydrates, and total lipids were stimulated by low concentrations of AgNPs [5-30 ppm].   On the other hand, the high concentrations of AgNPs [40–50 ppm] exhibited an inhibitory effect .The three antioxidant enzymes SOD, CAT, and GR were increased in a dose-dependent manner as a result of the increase in AgNPs concentrations. The total amino acids progressively increased under the same treatment. Electrophoretic polypeptide banding patterns consists of 159 bands with a molecular weight range of 27 to 145 kDa. Ten bands—represented polymorphic loci with the value of 6.21 %, whereas 6 bands—represented monomorphic loci with value of 3.77%. The most polypeptide bands [14 bands] were discovered in phycocyanin treatments at lane 5 with molecular weights ranging from 27 to 129 KDa as well as AgNPs treatments at lanes 9, 11 and 13 with a value reaching 8.80% and molecular weights ranging from 27 to 145 kDa.